Page 55 - 73_04

VOL. 73 (4), 833-871, 2007 ÁCIDOS SIÁLICOS: DISTRIBUCIÓN...

shed trans-sialidase activity from procyclic Trypanosoma congolense. Acta
Trop. 59. 117-29.
(48) SCHAUER, R. (2000) Achievements and challenges of sialic acid research.
Glycoconj. J. 17: 489-499.
(49) ZAPATA, G.; CROWLEY, J. M. Y VANN, W. F. (1992) Sequence and expresion of
the Escherichia coli NeuC gene product. J. Bacteriol. 174: 315-319.
(50) PETERSEN, M.; FESSNER, W.; FROSCH, M. Y LUNEBERG, E. (2000) The siaA gene
involved in capsule polisaccharide biosynthesis of Neisseria meningitidis B
codes for N-acylglucosamine-6-phosphate 2-epimerase activity. FEMS Mi-
crobiol. Lett. 184: 161-164.
(51) DAINES, D. A.; WRIGHT, L. F.; CHAFFIN, D. O.; RUBENS, C. E. Y SILVER, R. P.
(2000) NeuD plays a role in the synthesis of sialic acid in Escherichia coli
K1. FEMS Microbiol. Lett. 189: 281-284.
(52) SWARTLEY, J. S. Y STEPHENS, D. S. (1994) Identification of a genetic locus
involved in the biosynthesis of N-acetyl-D-mannosamine, a precursor of the
(alpha 2?8)-linked polysialic acid capsule of serogroup B Neisseria meningi-
tidis. J. Bacteriol. 176: 1530-1534.
(53) RODRIGUEZ-APARICIO, L. B.; FERRERO, M. A. Y REGLERO, A. (1995) N-acetyl-D-
neuraminic acid synthesis in Escherichia coli K1 occurs through condensa-
tion of N-acetyl-D-mannosamine and pyruvate. Biochem. J. 308: 501-505.
(54) FERRERO, M. A.; REGLERO, A.; FERNÁNDEZ-LÓPEZ, M.; ORDÁS, R. Y RODRIGUEZ-
APARICIO, L. B. (1996) N-acetyl-D-neuraminic acid lyase generates the sialic
acid for colominic acid biosynthesis in Escherichia coli K1. Biochem. J. 317:
157-165.
(55) BROSSMER, R.; ROSE, U.; KASPAR, D.; SMITH, T. L.; GRASMUK, H. Y UNGER, F. M.
(1980) Enzymic synthesis of 5-acetamido-9-azido-3,5,9-trideoxy-D-glycero-
D-galacto-2-nonulosonic acid, a 9-azido-9-deoxy derivative of N-acetylneura-
minic acid. Biochem. Biophys. Res. Commun. 96: 1282-1289.
(56) REGLERO, A.; FERNÁNDEZ-MARTÍNEZ, V.; GUTIÉRREZ, C. Y BRAVO, I. G. (2005) N-
acetylneuraminic acid (Neu5Ac) in bacteria: occurence, metabolism and
associated virulence. Current Topic in Biochem. Research. 7: 17-28.
(57) VIMR, E. R. Y LICHTENSTEIGER, C. (2002) To sialylate, or not sialylate: that is
the question. Trends in Microbiol. 10: 254-257.
(58) POPENOE, E.A. Y DREW, R.M. (1957) The action of an enzyme of Clostridium
perfringens on orosomucoid. J. Biol. Chem. 228: 673-683
(59) BRUG, J.; ESSER, R.J. Y PAERELS, G.B. (1959) The enzymic cleavage of N-
acetylneuraminic acid. Biochim. Biophys. Acta. 33: 241-242.
(60) UCHIDA, Y.; TSUKADA, Y. Y SUGIMORI, T. (1984) Purification and properties of
N-acetylneuraminate lyase from . J. Biochem. 96: 507-522.
(61) DEIJL, C.M. Y VLIEGENTHART, J.F. (1983) Configuration of substrate and pro-
ducts of N-acetylneuraminate pyruvate-lyase from Clostridium perfringens.
Biochem. Biophys. Res. Commun. 111: 668-674
(62) LILLEY, G.G.; BARBOSA, J.A.R.G., AND PEARCE, L.A. (1998) Expression in Esche-
richia coli of the putative N-acetylneuraminate lyase gene (nanA) from Hae-
mophilus influenzae: overproduction, purification, and crystallization. Pro-
tein Expression and Purification. 12: 295-304.

865