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5.*CONCLUSIONS*
1.?! The! optical! examination! of! the! lectin?binding! pattern! observed! under! a!
fluorescence! microscope! is! rapid! assay! that! allows! an! operative! typing! of! M.)
aeruginosa!colonies!directly!collected!from!the!environment!or!from!cultures.!!

2.?! The! lectin?binding! pattern! allows! differentiating! among! M.)aeruginosa! strains!
with! a! great! variety! in! toxin! production! isolated! from! different! blooms.! All! the! M.)
aeruginosa! colonies! from! the! same! strain! (as! well! as! all! the! cells! of! the! same!
colony)!showed!similar!labeling.!This!lectin?binding!pattern!of!each!strain!is!close!
related!with!its!microcystin!production.!A!cluster!classified!based!on!lectin!binding!
pattern! separates! M.) aeruginosa! strains! into! three! different! groups:! non?toxic,!
moderate!toxicity!and!high!toxicity.!

!3.?! Non?toxic! M.) aeruginosa! strains! were! positively! bound! with! UEA?1! lectin.! In!
contrast,! the! most! toxic! strains! remain! unbound.! This! procedure! also! was!
successful!in!field!samples!from!M.)aeruginosa!blooms.!

6.*ACKNOWLEDGEMENTNS*
This! work! has! by! been! financially! supported! by! the! Spanish! Ministry! of!

Sciences! and! Innovation! through! the! grants! CTM2008?! 05680! C02?01/MAR! and!
CGL2008?00652/BOS.! Authors! thank! the! financial! support! given! the! project!
CENIT?VIDA! and! by! the! Ministerio! de! Educación! y! Ciencia,! through! FPU! program.!
Special!thanks!are!given!Lara!de!Miguel!Fernandez!by!technical!support!

7.*REFERENCES*

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2.! Gorham,! P.R.;! Carmichael,! W.W.;! In! Algae! and! human! affairs.! Lembi,! C.A.,! Waaland,! J.R.,! Eds.;!
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3.!Carmichael,!W.W.!(1992).!J.!of!Appl.!Microb.,!72,!445.!
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