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VOL. 72 (3), 519-538, 2006 NEW PERSPECTIVES IN OCULAR PHARMACOLOGY...
In vitro experiments
In order to understand the molecular mechanism that make
corneal epithelial cells to migrate faster after the challenge with
some nucleotides and dinucleotides, experiments were performed
with corneal epithelial cells in primary cultures (Figure 2A).
FIGURE 2. Effect of dinucleotides on corneal wound healing. A. Corneal
epithelial cells were isolated and seeded as primary cultures which can be observed
under Normaski optics and provide positive labelling to the P2Y2 receptor
antibody. B. Diadenosine polyphosphates were able modify the rate of
re-epithlialization, Ap4A being the best dinucleotide in its ability to accelerate
cell migration.
The treatment of epithelial cells with diadenosine polyphosphates
after scratching the monolayer with a pipette tip, demonstrated that
as occur in vivo, Ap4A was able to accelerate the rate of healing while
Ap3A and Ap5A clearly delayed the rate of healing (Figure 2B). Other
nucleotides, such as ADP did not modify the rate of healing when
compared to control. In both cases, a positive or negative effect on
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