Anales RANF

S4-03 ATP-INDUCED CALCIUM SIGNALLING IN MESENCHYMAL STEM CELL FUNCTIONS Lin-Hua Jiang School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, UK. Mesenchymal multipotent stem cells (MSCs) have many promising applications in tissue regeneration and cell-based therapies, and a good understanding of how extracellular chemical, physical and biological signals regulates MSC functions should provide useful information for better applications of MSC. ATP is one of the most commonly-used autocrine and paracrine signalling molecules and can interact with the ligand-gated P2X receptors and G-protein-coupled P2Y receptors to raise the level of cytosolic calcium. Increasing evidence shows that MSCs release ATP in vitro and in vivo. I will present our own study aimed to elucidate the molecular mechanisms that participate in ATP-induced calcium signalling in human dental pulp-derived MSC. I will also discuss the role of such calcium signalling mechanisms and downstream calcium-dependent signal transduction pathways in ATP-induced regulation of cell migration and differentiation. References: [1] Peng, H.S., Hao, Y.J., Mousawi,F., Roger, S., Li, J., Sim, J. A., Ponnambalam, S., Yang, X.B., and Jiang, L.-H. (2016) Purinergic and store-operated Ca 2+ signalling mechanisms in mesenchymal stem cells and their roles in ATP-induced stimulation of cell migration. Stem Cells 34: 2102-2114. [2] Jiang, L.-H., Hao, Y.J., Mousawi, F., Peng, H.S. and Yang, X.B. (2017) Expression of P2 purinergic receptors in mesenchymal stem cells and their roles in extracellular nucleotide regulation of cell functions. Journal of Cell Physiology 232: 287-297.