Anales RANF

P.33 IDENTIFICATION OF P2X7-DEPENDENT TRANSCRIPTOME AFTER SEIZURES Conte G. 1 , Jimenez-Mateos E. 2 , Engel T 1 . 1 Royal College of Surgeons in Ireland, Dublin (Ireland); 2 Trinity College, Dublin (Ireland). Current epilepsy treatments are targeting excitatory or inhibitory pathways without any disease-modifying effect, moreover these therapies are ineffective in over 30% of patients, suggesting a need to investigate new targets with different mechanisms of actions. The ATP-gated P2X7 receptor, implicated in seizure generation and driving inflammatory processes, has recently been shown to be a promising target for epilepsy, showing anticonvulsant and neuroprotective properties in animal models. However, there are important gaps in our knowledge about the mechanism of action of this receptor that must be filled. P2X7 down-stream targets must be identified to establish accessible biomarkers to predict a pathological P2X7 activation in the brain. Mounting evidences have showed that microRNA may be a key player in the pathogenesis of epilepsy, suggesting not only a therapeutic but also a diagnostic potential of microRNAs. Therefore, the aim of our study is to identify the P2X7 dependent transcriptome after status epilepticus focusing on microRNA changes. For this purpose we used intraamigdala injection of kainic acid model to induce status epilepticus in two groups of mice, P2X7 knock out and wild type. We collected the hippocampus 8 hours after the status epilepticus and extract the mRNA to perform miRNA open array. We found significant changes in knock out mice when compared to wild type in several miRNA, suggesting that P2X7 is involved in transcriptional changes during seizures.