Anales RANF

S17-03 PURINERGIC SIGNALING IN INFLAMMATORY BOWEL DISEASE AND IT’S ROLE IN THE EFFECT OF ACUPUNCTURE AND MOXIBUSTION Qiaofeng Wu*, Weishang Hu, Sihui Li Acupuncture and Moxibustion College, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 610075, China; • Background or Introduction Inflammatory bowel disease (IBD) is a chronic, relapsing, inflammatory disease of the gut. The exact cause of IBD is still unknown. A growing body of evidence indicates that purinergic signaling and its receptor system play important roles in IBD. • Material and methods Dextran Sulfate Sodium (DSS) induced colitis mice were set up and electro- acupuncture and moxibustion were used to treated the mice. The expression of CD39, CD73 and A2a proteins in the colon were detected by westernblot and the levels of CD4+CD39+ T cells, CD4+CD39+Foxp3+ T cells, CD4+CD73+ T cells and CD4+CD73+Foxp3+ T cells in peripheral blood, spleen, and local draining lymph node were detected by flowcytometry. • Results The protein expression of CD39, CD73, and A2a , the number of positive cells in the colons were decreased in the colitis model group. After acupuncture and moxibustion treatment, the above detections were significantly improved. The number of CD4+CD39+T cells, CD4+CD73+ T cells in the peripheral blood, lymph nodes and spleen of mice were all decreased in the model mice. However, after eletro-acupucture and moxibustion treatment, both of the number of CD4+CD39+T cells and CD4+CD73+ T cells significantly improved. Additionally, the ratio of CD4+ Foxp3+CD39+ T cells to CD4+CD39+T cells,and the ratio of CD4+ Foxp3+CD73+ T cells to CD4+CD73+T cells in the peripheral blood, lymph nodes and spleen of mice were all increased after treatment (all P values were less than 0.01). • Conclusions Both moxibustion and electro-acupuncture have good therapeutic effects on DSS- induced colits mice. Regulation of Treg cells may be one of the underline mechanisms of acupuncture and moxibustion. Key words: Colitis, Electro-acupuncture,Moxibustion , Adenosine metabolic pathway, CD39 ,CD73 ,A2a