Anales RANF

Juan Aparicio Blanco, Ignacio A. Romero, Jean P Benoit, Ana I. Torres Suárez @Real Academia Nacional de Farmacia. Spain 206 Figure 3: Quantitative analysis by flow cytometry of influence of particle size reduction and functionalization with CBD on the in vitro BBB (left) and glioma (right) targeting ability of LNCs. *: p<0.05, **: p<0.01, ***: p<0.001. Figure 4: 3D reconstructions with the IMARIS software of the Z-stacks of confocal images with hCMEC/D3 cells: undecorated (left) and CBD-adsorbed (right) fluorescently-labeled LNCs. Scale bar = 10 µm. Importantly, we verified that the nanocapsules themselves did not alter the barrier properties of the hCMEC/D3 monolayer before conducting the BBB permeability experiments with LNCs. Effectively, there were no statistically significant differences between the permeability coefficients of TRITC-dextran across the hCMEC/D3 monolayer in the presence and the absence of LNCs (1.67 ± 0.44 x 10 -7 cm/s versus 1.77 ± 0.33 x 10 -7 cm/s, p>0.05). These results demonstrated the integrity of the BBB model throughout the 24 hours period evaluated and consequently the suitability of this model for evaluating the BBB transport ability of the LNCs. The quantitative analysis of the in vitro BBB- permeability coefficients is shown in Figure 5. We have used herein the permeability coefficient as a robust parameter that readily enables the comparison of transport